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quantigene singleplex assay kit  (Thermo Fisher)


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    Thermo Fisher quantigene singleplex assay kit
    Quantigene Singleplex Assay Kit, supplied by Thermo Fisher, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/result/quantigene singleplex assay kit/product/Thermo Fisher
    Average 90 stars, based on 1 article reviews
    quantigene singleplex assay kit - by Bioz Stars, 2026-03
    90/100 stars

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    Jurkat cells were treated with siRNAs targeting WAPAL (A and D), AURKA (B and E), or JAK1 (C and F) at the concentrations indicated below the x-axis. Cells were either harvested 6 days post-treatment (D-F) or sampled serially from siRNA-treated cells on the days indicated on the x-axis (A-C). The sampled cells were lysed and frozen. All samples were subjected to QuantiGene Singleplex assays simultaneously for mRNA quantification. IC50 values were determined using the log (inhibitor) – three parameters function in GraphPad Prism. Data points at different time points and treatments were compared using two-way ANOVA with multiple comparison corrections. * p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, N=3-7, mean ± SEM

    Journal: bioRxiv

    Article Title: Structure – silencing duration relationships in RNAi medicines in rapidly dividing cells

    doi: 10.1101/2024.09.09.612002

    Figure Lengend Snippet: Jurkat cells were treated with siRNAs targeting WAPAL (A and D), AURKA (B and E), or JAK1 (C and F) at the concentrations indicated below the x-axis. Cells were either harvested 6 days post-treatment (D-F) or sampled serially from siRNA-treated cells on the days indicated on the x-axis (A-C). The sampled cells were lysed and frozen. All samples were subjected to QuantiGene Singleplex assays simultaneously for mRNA quantification. IC50 values were determined using the log (inhibitor) – three parameters function in GraphPad Prism. Data points at different time points and treatments were compared using two-way ANOVA with multiple comparison corrections. * p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, N=3-7, mean ± SEM

    Article Snippet: QGS assay (Invitrogen™QuantiGene™ Sample Processing Kit, cultured cells (QS0103, Life Technologies GmbH), Invitrogen™QuantiGene™ Singleplex Assay Kit (QS0016, Life Technologies GmbH)) was performed according to manufacturer’s protocol.

    Techniques: Comparison

    Jurkat cells underwent repeated treatments with siRNAs targeting WAPAL at concentrations of either 1 µM (A) or 2 µM (B), up to a maximum of three doses, administered 3 days apart. Cells were harvested from the siRNA-treated cultures on the days indicated on the x-axis, then lysed and frozen. All samples were analyzed simultaneously for mRNA quantity using the QuantiGene Singleplex assay. Data points at different time points and treatments were compared using two-way ANOVA with multiple comparison corrections. * p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, N=3, mean ± SEM

    Journal: bioRxiv

    Article Title: Structure – silencing duration relationships in RNAi medicines in rapidly dividing cells

    doi: 10.1101/2024.09.09.612002

    Figure Lengend Snippet: Jurkat cells underwent repeated treatments with siRNAs targeting WAPAL at concentrations of either 1 µM (A) or 2 µM (B), up to a maximum of three doses, administered 3 days apart. Cells were harvested from the siRNA-treated cultures on the days indicated on the x-axis, then lysed and frozen. All samples were analyzed simultaneously for mRNA quantity using the QuantiGene Singleplex assay. Data points at different time points and treatments were compared using two-way ANOVA with multiple comparison corrections. * p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, N=3, mean ± SEM

    Article Snippet: QGS assay (Invitrogen™QuantiGene™ Sample Processing Kit, cultured cells (QS0103, Life Technologies GmbH), Invitrogen™QuantiGene™ Singleplex Assay Kit (QS0016, Life Technologies GmbH)) was performed according to manufacturer’s protocol.

    Techniques: Singleplex Assay, Comparison

    HeLa cells were exposed to siRNAs targeting WAPAL (A and D), AURKA (B and E), or JAK1 (C and F) at the concentrations shown below the x-axis. Cells were either collected 6 days after treatment (D-F) or sampled periodically from siRNA-treated cells on the days indicated on the x-axis (A-C). The collected cells were lysed and frozen. All samples underwent simultaneous mRNA quantification using QuantiGene Singleplex assays. IC50 values were calculated using the log (inhibitor) – three parameters function in GraphPad Prism. Data points from various time points and treatments were compared using two-way ANOVA with multiple comparison corrections. * p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, N=3-9, mean ± SEM

    Journal: bioRxiv

    Article Title: Structure – silencing duration relationships in RNAi medicines in rapidly dividing cells

    doi: 10.1101/2024.09.09.612002

    Figure Lengend Snippet: HeLa cells were exposed to siRNAs targeting WAPAL (A and D), AURKA (B and E), or JAK1 (C and F) at the concentrations shown below the x-axis. Cells were either collected 6 days after treatment (D-F) or sampled periodically from siRNA-treated cells on the days indicated on the x-axis (A-C). The collected cells were lysed and frozen. All samples underwent simultaneous mRNA quantification using QuantiGene Singleplex assays. IC50 values were calculated using the log (inhibitor) – three parameters function in GraphPad Prism. Data points from various time points and treatments were compared using two-way ANOVA with multiple comparison corrections. * p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, N=3-9, mean ± SEM

    Article Snippet: QGS assay (Invitrogen™QuantiGene™ Sample Processing Kit, cultured cells (QS0103, Life Technologies GmbH), Invitrogen™QuantiGene™ Singleplex Assay Kit (QS0016, Life Technologies GmbH)) was performed according to manufacturer’s protocol.

    Techniques: Comparison

    Primary human T cells were treated with siRNAs targeting WAPAL (A and D), AURKA (B and E), or JAK1 (C and F) at the concentrations indicated below the x-axis. Cells were either harvested 6 days post-treatment (D-F) or sampled at intervals from siRNA-treated cells on the days indicated on the x-axis (A-C). The harvested cells were lysed and frozen. All samples were simultaneously analyzed for mRNA levels using QuantiGene Singleplex assays. IC50 values were determined using the log (inhibitor) – three parameters function in GraphPad Prism. Data points from different time points and treatments were compared using two-way ANOVA with multiple comparison corrections. * p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, N=3-7, mean ± SEM

    Journal: bioRxiv

    Article Title: Structure – silencing duration relationships in RNAi medicines in rapidly dividing cells

    doi: 10.1101/2024.09.09.612002

    Figure Lengend Snippet: Primary human T cells were treated with siRNAs targeting WAPAL (A and D), AURKA (B and E), or JAK1 (C and F) at the concentrations indicated below the x-axis. Cells were either harvested 6 days post-treatment (D-F) or sampled at intervals from siRNA-treated cells on the days indicated on the x-axis (A-C). The harvested cells were lysed and frozen. All samples were simultaneously analyzed for mRNA levels using QuantiGene Singleplex assays. IC50 values were determined using the log (inhibitor) – three parameters function in GraphPad Prism. Data points from different time points and treatments were compared using two-way ANOVA with multiple comparison corrections. * p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, N=3-7, mean ± SEM

    Article Snippet: QGS assay (Invitrogen™QuantiGene™ Sample Processing Kit, cultured cells (QS0103, Life Technologies GmbH), Invitrogen™QuantiGene™ Singleplex Assay Kit (QS0016, Life Technologies GmbH)) was performed according to manufacturer’s protocol.

    Techniques: Comparison

    Jurkat cells were treated with siRNAs that were either simply phosphorylated or stabilized with 5’-VP at the 5’ end of the antisense strand. The siRNAs targeted WAPAL (A and C) or AURKA (B and D) at concentrations of 2 µM (A-B) or 4 µM (C-D). Samples were periodically collected from the treated cultures as indicated on the x-axis. These samples were frozen and later thawed simultaneously for mRNA quantification using QuantiGene Singleplex assays. Data points from various time points and treatments were compared using two-way ANOVA with multiple comparison corrections. N=3, mean ± SEM

    Journal: bioRxiv

    Article Title: Structure – silencing duration relationships in RNAi medicines in rapidly dividing cells

    doi: 10.1101/2024.09.09.612002

    Figure Lengend Snippet: Jurkat cells were treated with siRNAs that were either simply phosphorylated or stabilized with 5’-VP at the 5’ end of the antisense strand. The siRNAs targeted WAPAL (A and C) or AURKA (B and D) at concentrations of 2 µM (A-B) or 4 µM (C-D). Samples were periodically collected from the treated cultures as indicated on the x-axis. These samples were frozen and later thawed simultaneously for mRNA quantification using QuantiGene Singleplex assays. Data points from various time points and treatments were compared using two-way ANOVA with multiple comparison corrections. N=3, mean ± SEM

    Article Snippet: QGS assay (Invitrogen™QuantiGene™ Sample Processing Kit, cultured cells (QS0103, Life Technologies GmbH), Invitrogen™QuantiGene™ Singleplex Assay Kit (QS0016, Life Technologies GmbH)) was performed according to manufacturer’s protocol.

    Techniques: Comparison

    Jurkat cells were treated with siRNAs stabilized with 5’-VP and targeting PPIB at various concentrations as indicated in the color code (2 µM in orange, 1 µM in dark blue, 0.5 µM in light blue, A-B) or on the x-axis (C-D). The siRNAs were covalently conjugated to either a divalent myristic acid moiety (A and C) or cholesterol (B and D). Cells were either harvested 6 days post-treatment (B and D) or sampled periodically as indicated on the x-axis (A and C). Samples were frozen, later thawed, and mRNA was quantified using QuantiGene Singleplex assays. Data points from various time points and treatments were compared using two-way ANOVA with multiple comparison corrections. * p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, N=3-6, mean ± SEM

    Journal: bioRxiv

    Article Title: Structure – silencing duration relationships in RNAi medicines in rapidly dividing cells

    doi: 10.1101/2024.09.09.612002

    Figure Lengend Snippet: Jurkat cells were treated with siRNAs stabilized with 5’-VP and targeting PPIB at various concentrations as indicated in the color code (2 µM in orange, 1 µM in dark blue, 0.5 µM in light blue, A-B) or on the x-axis (C-D). The siRNAs were covalently conjugated to either a divalent myristic acid moiety (A and C) or cholesterol (B and D). Cells were either harvested 6 days post-treatment (B and D) or sampled periodically as indicated on the x-axis (A and C). Samples were frozen, later thawed, and mRNA was quantified using QuantiGene Singleplex assays. Data points from various time points and treatments were compared using two-way ANOVA with multiple comparison corrections. * p<0.05, **p<0.01, ***p<0.001, ****p<0.0001, N=3-6, mean ± SEM

    Article Snippet: QGS assay (Invitrogen™QuantiGene™ Sample Processing Kit, cultured cells (QS0103, Life Technologies GmbH), Invitrogen™QuantiGene™ Singleplex Assay Kit (QS0016, Life Technologies GmbH)) was performed according to manufacturer’s protocol.

    Techniques: Comparison

    HeLa cells were initially transduced with a miR-155-expressing lentivirus and then transfected with a DualGlo plasmid containing the miR-155 target sequence cloned four times in tandem into the 3’ UTR of Rluc. The transfection was repeated once a week. The transduced and transfected cells were treated with a cholesterol-conjugated miR-155 inhibitor (A) and luminescence was measured using the DualGlo assay. The Rluc signal was normalized to the Fluc signal. Jurkat cells were also treated with the cholesterol-conjugated miR-155 inhibitor, and the miR-155 target, MYD88 mRNA, was quantified using the QuantiGene Singleplex assay on the days indicated on the x-axis (B). Data curves from various time points and treatments were compared using two-way ANOVA. N=3-10, mean ± SEM

    Journal: bioRxiv

    Article Title: Structure – silencing duration relationships in RNAi medicines in rapidly dividing cells

    doi: 10.1101/2024.09.09.612002

    Figure Lengend Snippet: HeLa cells were initially transduced with a miR-155-expressing lentivirus and then transfected with a DualGlo plasmid containing the miR-155 target sequence cloned four times in tandem into the 3’ UTR of Rluc. The transfection was repeated once a week. The transduced and transfected cells were treated with a cholesterol-conjugated miR-155 inhibitor (A) and luminescence was measured using the DualGlo assay. The Rluc signal was normalized to the Fluc signal. Jurkat cells were also treated with the cholesterol-conjugated miR-155 inhibitor, and the miR-155 target, MYD88 mRNA, was quantified using the QuantiGene Singleplex assay on the days indicated on the x-axis (B). Data curves from various time points and treatments were compared using two-way ANOVA. N=3-10, mean ± SEM

    Article Snippet: QGS assay (Invitrogen™QuantiGene™ Sample Processing Kit, cultured cells (QS0103, Life Technologies GmbH), Invitrogen™QuantiGene™ Singleplex Assay Kit (QS0016, Life Technologies GmbH)) was performed according to manufacturer’s protocol.

    Techniques: Transduction, Expressing, Transfection, Plasmid Preparation, Sequencing, Clone Assay, Singleplex Assay